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Image Search Results
Journal: Journal of Applied Physiology
Article Title: Fetal sex and the relative reactivity of human umbilical vein and arteries are key determinants in potential beneficial effects of phosphodiesterase inhibitors
doi: 10.1152/japplphysiol.00540.2023
Figure Lengend Snippet: Relative protein amounts for several IBMX-sensitive phosphodiesterase (PDE) isoforms in human umbilical arteries. F, females; M, males. Relative protein content of PDE5A ( A ), PDE1B ( B ), PDE1C ( C ), PDE4B ( D ), and PDE3A ( E ) was evaluated by Western blot in human umbilical artery (HUA) homogenates of appropriate for gestational age (AGA) and intrauterine growth restriction (IUGR) newborns. For each experimental group, samples from 40 patients were randomly distributed into 4 pools. For each pool, the target protein amount was normalized to the corresponding Ponceau S total protein staining and reported to the amount measured in a “standard” sample. Data are presented as individual values, with line at means ± SE ( n = 4 pools of 10 patients). P values were obtained using a Mann-Whitney test to compare AGA and IUGR neonates. A representative Western blot is shown at top .
Article Snippet: After the staining was removed, membranes were blocked in casein solution (Vector, cat. no. SP-5020) (30 min, at room temperature) and immunoblotted (overnight, 4°C) using specific antibodies against sGC (1:2,000, Abcam, cat. no. ab50358, RRID:AB_880325), PKG (1:1,000, Enzo Life Sciences, cat. no. ADI-KAP-PK005-D, RRID:AB_2039483), PDE1B (1:2,000, OriGene, cat. no. TA503319, RRID:AB_11125416), PDE1C (1:250, GeneTex, cat. no. GTX14602, RRID:AB_368824), PDE3A (1:1,000, GeneTex, cat. no. GTX112305, RRID:AB_11168131), PDE4B (1:1,000, GeneTex, cat. no. GTX113798, RRID:AB_11173431), or
Techniques: Western Blot, Staining, MANN-WHITNEY